Please forward this error screen to sharedip-160153402. Department of Biomedical Engineering, Boston University, 44 Cummington Street, Boston, MA 02215, United States of America. HIV infection methods of diagnosing children the basis of HIV antibody detection.
T-cell count and thereby determine when to initiate ART. These technologies are useful in a broad spectrum of resource-limited settings but they have disadvantages. With the exception of rapid tests, the technologies are costly, take hours to perform and require highly skilled technicians and dedicated laboratory spaces. As a result, many developing countries are unable to meet the demand for HIV infection diagnosis and monitoring.
On the basis of these considerations, it is not surprising that in some locations little or no overlap exists between the set of HIV-associated health-care technologies that meet a specific need and the set of technologies that are practical and affordable. Therefore, a more ideal suite of technologies for use at the point of care is called for, especially in resource-limited settings. Tools that satisfy the ASSURED criteria primarily aim to provide same-day diagnosis and facilitate immediate decision-making. These include well established standard devices, those in the pipeline and those currently being researched. We focus only on low-cost devices that are free of the limitations associated with the use of gold standard laboratory tests in resource-limited areas. 10 The most ready-to-use types of rapid tests are lateral flow tests, which do not require reagents.
Fear of stigmatization from testing positive for HIV can deter at-risk individuals from seeking consultation17 and can make people reluctant to disclose their HIV status. The use of saliva and urine specimens for rapid testing offers a discrete alternative to blood-based tests in settings where stigma, lack of education, cultural practices and privacy concerns undermine HIV prevention. These testing algorithms improve the positive predictive value of rapid tests in populations with a low prevalence of HIV infection, in which false-positive results are more common. 28 As such, commercial assays for measuring this protein are cheap alternatives for virus load monitoring in low-resource settings. Several reports support the use of the EXAVir Load Version 3 nucleic acid test for HIV load monitoring in the developing world. 31 In this test, a gel-separation step isolates virions from plasma components. The virions are then lysed and the lysates undergo a modified ELISA to measure the activity of reverse transcriptase.
T-cell count,28 and thus early generation p24 immunoassays offered low-cost options for measuring HIV load. 24 immunoassays are no longer considered for this activity, however, because of their low sensitivity, the high variability of the results between assays and the difficulty in standardizing the correlation between p24 and virus load in persons receiving ART. HIV tests with high sensitivity would be enormously helpful to the thousands of infants at risk in countries where the distribution of PCR machines is limited. Development of p24 rapid tests has focused mainly on improving sensitivity.